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Stem Cells for Acne Scar Repair (SCIENCE FACT, not science fiction!)

 
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(@holdontohope)

Posted : 01/28/2009 12:53 am

Well, I wasn't posting this to insult you, your level of intelligence, or anyone else agreeing with you on this topic; but I'll let you feel better about that, I could care less what you think of me.

All that I was trying to point out honestly, is how rediculous this sounds. At what point or cost will you go to get rid of your acne and/or scars?? This is kind of similar to the Hollywood stars that paid thousands of dollars when Liposuction was a new miracle procedure and cost 10X what it does now... Back then it seemed to be the perfect answer to a simple problem, and look at it now..

Stem cell research is still a HUGE morality issue ALL AROUND.. no matter which way you look at it, for many different reasons. It's reguardless of the type of ailment it's hoping to help... OR how the procedue is done or in which manner...

But if you want to take those cells from your stomach and combine them with fat cells, have them cultured in a petri dish just to have a few scars minimized.. Go ahead. I just joined acne.org and love this site... But this, is simply rediculous.

I'm just like everyone else on here when it comes to my acne. But I don't believe in the miracle cures out there, and I don't want false hope. I'm not going to spend hundreds of dollars on something that isn't guaranteed to work... and I don't want some doctor playing god with my skin... Yes I get depressed too and I'd do anything to have beautiful skin... but within a reasonable explanation or method.

Look, Kate, let's not get into an argument over feelings. Emotions run high on this board! If you want to tell people to stop pursuing the use of stem cells for acne scarring, well, good luck. C'mon and do yourself a favor and try and only contribute meaningful posts that will help us find solutions. That's what this board is for. That's why we're here.

Moving on.......

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(@Anonymous)

Posted : 01/28/2009 1:23 am

Kate, I'm not sure you realize how much some people are suffering because of their acne scars. Why do you feel it's ridiculous for people to use their own stem cells to repair their skin? If anyone is lucky enough to afford the treatment I say more power to them!

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(@2012)

Posted : 01/28/2009 2:34 am

Either way, by culturing the said stem cells for a short period of time, one can increase their numbers exponentially. It really isn't very hard to increase the number of stem cells found in 10cc

of fat tissue 1000 fold. For example, a thousand cells can be turned into a million cells with the right culturing solution, incubation temperature and time management. Think of what a 1000X viable cell mass can do for the skin in comparison to what has already been achieved with the traditional fat transfer procedure...

BRD

BRD,

I want to say a heartfelt THANK YOU for your COMPASSION, TIME, and ENERGY put into informing us the latest in Stem Cell medical technology for scar remodeling. This is really the most promising treatment for acne scaring I have heard in a VERY long time. You are a great treasure on this board and we are so fortunate to have you with us!

Please stay with us and keep us informed with your latest update.

I am considering the treatment at SEISHIN Regenerative Medicine Center in Japan.

http://www.saisei-iryou.com/english/cellantiaging/

But after reading about the procedure outlined on their website, I have one question.

I understand that The Celution System allows automated, precise separation and extraction of adipose tissue-derived stem cells... However, there was NO mention on their website that, after the extraction, the cells are then cultured for a period of time to increase their numbers exponentially... in order to achieve the maximum result in regeneration.

In fact, it even appears that in SEISHIN, the extracted stem cells are (or can be) injected to the scar immediately after its extraction (thus leaving no time for culturing).

In reading your introduction (your first post) on mysenchymal stem cells, I was under the impression that culturing the mysenchymal stem cells to increase its number by 1000s is an essential step of this procedure to yield maximum result in scar remodeling.

In your opinion, Is Seishin missing this important step? I would think unlikely. But why this step of culturing to increase numbers of mysenchymal stem cells not described in their procedure?

Or maybe I am missing something here? For example, can the Celution not only extract but also culture the mysenchymal stem cells to increase its numbers?

I can imagine your inbox must be flooded with questions. But I hope I hear from you. THANK YOU.

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(@thelegend)

Posted : 01/28/2009 9:56 am

Well, I wasn't posting this to insult you, your level of intelligence, or anyone else agreeing with you on this topic; but I'll let you feel better about that, I could care less what you think of me.

All that I was trying to point out honestly, is how rediculous this sounds. At what point or cost will you go to get rid of your acne and/or scars?? This is kind of similar to the Hollywood stars that paid thousands of dollars when Liposuction was a new miracle procedure and cost 10X what it does now... Back then it seemed to be the perfect answer to a simple problem, and look at it now..

Stem cell research is still a HUGE morality issue ALL AROUND.. no matter which way you look at it, for many different reasons. It's reguardless of the type of ailment it's hoping to help... OR how the procedue is done or in which manner...

But if you want to take those cells from your stomach and combine them with fat cells, have them cultured in a petri dish just to have a few scars minimized.. Go ahead. I just joined acne.org and love this site... But this, is simply rediculous.

I'm just like everyone else on here when it comes to my acne. But I don't believe in the miracle cures out there, and I don't want false hope. I'm not going to spend hundreds of dollars on something that isn't guaranteed to work... and I don't want some doctor playing god with my skin... Yes I get depressed too and I'd do anything to have beautiful skin... but within a reasonable explanation or method.

At what point or cost would I go to get rid of my acne scars...well lets see....being disfigured looking until the day I die isnt giving me a lot to look forward to so I'll say a pretty long way....I dont know...I guess a $30,000 lump sum or maybe $1,000 a year until I die sounds ok with me to NOT BE DISFIGURED!!!

What would I rather have....$30,000 and be disfigured looking until my flesh rots off in a grave or...NOT BE DISFIGURED LOOKING AND FINALLY HAVE A CHANCE AT HAPPINESS AND NOT FEELING ASHAMED OF MYSELF FOR SOMETHING I HAD NOTHING TO DO WITH and NO CONTROL OVER!!!

decisions...decisions

This is not at all similar to people getting Liposuction because Liposuction is the easy way out for fat people that have let themselves go over a period of a lifetime and their obesity in most cases are the result of their own lifestyles because they refuse to do whats right for themselves and live healthy lives. Most of these people r fat because they DESERVE to be fat as a consequence of their lifestyle that THEY HAVE CHOSEN...I know in all cases of obesity this is not true but in MOST it IS....we who have acne scars NEVER HAD A CHOICE...IT WAS NEVER OUR FAULT...we have had to suffer through the hopelessness of actually doing everything right....living a more healthy life than those who do not have acne or scars to try to fix ourselves and in return for our efforts all we get is failure....so why in the hell would we not want to do this procedure at whatever the cost?

In many cases morality has SUFFICATED the advancement and betterment of man kind for thousands of years...just think of where we would be today if we had not been chained by its ignorance...if you knew anything about the past and the way religion dealt with IMMORAL people you would know that countless intelligent, innocent people were killed by these MORAL people out of fear, ignorance and words from a book that they themselves did not fully understand.

I'm fine with a human being playing God with my skin to heal me...because I'm tired of waiting on God to do it himself....you can pray to have your scars healed while everyone else actually tries to have something done about it and see who gets healed first

I'm perfectly fine with humanity playing god or even becoming IT because I am tired of living down on my knees and seeing so many suffer and have to accept it because they think its the way god wanted it to be

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(@heidi55)

Posted : 01/28/2009 10:54 am

Amen!! I am going to see this doctor tomorrow so will report back on Friday morning. I have moderate scarring. Fingers crossed!! I, we, dont deserve this...

 

I will let you guys know tomorrow how it goes...

Heidi

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(@franklins-tower)

Posted : 01/28/2009 11:05 am

Amen!! I am going to see this doctor tomorrow so will report back on Friday morning. I have moderate scarring. Fingers crossed!! I, we, dont deserve this...

I will let you guys know tomorrow how it goes...

Heidi

Which doctor is that - Dr. Khan?

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(@heidi55)

Posted : 01/28/2009 11:17 am

yes - the same doctor as icaretohelp

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(@datura)

Posted : 01/28/2009 12:03 pm

yes - the same doctor as icaretohelp

Heidi, I thought I heard you mention that you were going to see Dr. Sinclair in Australia. Either way, good luck! And keep us posted!

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(@franklins-tower)

Posted : 01/28/2009 12:10 pm

BRD,

 

Do you think that autologous adipose derived mesenchymal stem cells will improve hypertrophic scars even without an excision or use of ECM components?

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(@heidi55)

Posted : 01/28/2009 12:12 pm

HI

 

 

Thanks for following my threads...

 

I live in London, but have family in Australia. Dr Sinclair is a great doc but tf this doc can offer something better for me I will go with him as I live locally.

 

I am looking for the best solution as I want to put this journey behind me once and for all. I just want to get on with my life now and focus on better skin and being happy...Fingers crossed.

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(@foxclose)

Posted : 01/28/2009 2:06 pm

I think I'll wait for before/after pics. No doubt "cultured stem cells" does sound interesting.

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(@mclarensupra)

Posted : 01/28/2009 2:25 pm

Well its worth a shot to ask..is there any way this treatment is available in the U.S.?

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(@franklins-tower)

Posted : 01/28/2009 3:35 pm

BRD,

 

If using autologous adipose derived mesenchymal stem cells would it still be necessary or significantly valuable to also stimulate adult bone marrow to produce more stem cells?

 

Heidi,

 

Can you ask Dr. Khan if he would use TGF b-3 "to regulate stem cells, fibroblasts, etc during healing"?

 

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(@deadliest-catch)

Posted : 01/28/2009 9:47 pm

http://www.christopherreeve.org/site/c.mtK...Center_Home.htm

 

Chrisropher reeves RIP, this site shows latest stem cell updates.

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(@bulgarian-r)

Posted : 01/29/2009 10:27 pm

(EDIT)

 

Post reposted below 2012's post. You will find it if you scroll down.

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(@2012)

Posted : 01/29/2009 10:48 pm

First and foremost, I am not going to go into academic detail about the use of progenitor cells, particularly autologous adipose derived mesenchymal stem cells, for the treatment of various skin conditions including the repair of hypotrophic (depressed) acne scars. I realize most people here will not be interested in me blabbing on and on about this and that hypothesis while citing various academic articles. I am simply going to say that I am preparing to conduct an experiment with these cells as I believe they are far more capable of flattening and beautifying skin in comparison to anything else that is currently used by the conformist medical establishment.

Anyway, both anecdotal and peer reviewed evidence exists to suggest that mesenchymal stem cells can be used effectively to radically alter the appearance of one's "damaged" skin. The anecdotal evidence largely comes from people who have had successful autologous fat transfers. If the procedure is done right, a large number of adipocytes and resident stem cells can survive the transfer only to integrate themselves into the site of injection. When this happens, the patient often observes a very positive and noticeable difference in their skin within a few weeks/months time.

However, there are not that many mesenchymal stem cells in fat. So, some researchers have proposed a more advanced fat transfer technique, wherein the mesenchymal stem cells are separated from the fat cells, then cultured/multiplied for a few days/weeks, and then either (A) recombined with the fat cells and injected into facial tissue deficits, or (B) injected without recombination with fat cells.

Either way, by culturing the said stem cells for a short period of time, one can increase their numbers exponentially. It really isn't very hard to increase the number of stem cells found in 10cc of fat tissue 1000 fold. For example, a thousand cells can be turned into a million cells with the right culturing solution, incubation temperature and time management. Think of what a 1000X viable cell mass can do for the skin in comparison to what has already been achieved with the traditional fat transfer procedure...

OK, to make a long story short I want to try this procedure in 2009. Currently I am in the process of adding new equipment to my lab. Once that's done and I am brave enough to try some of my ideas I will go for it.

That said, I am most certainly not the first person to be doing this. There are many clinics in Eastern Europe and East Asia that are already offering this service. I don't know much about their quality control processes nor have I seen their results firsthand. However, a colleague recently sent me some before/after pictures of a doctor in Asia who is apparently trying the above described type (A) procedure. Again, what this means is that stem cells are extracted from fat tissue, then cultured, and finally recombined with the fat tissue a few days later into what is essentially an enhanced autologous cellular mixture which can regenerate tissue deficits present at the site of acne scars... This doctor is experimenting with stem cells for anything from scar revision to breast enlargement.

Anyway, here are the photos that I received:

Acne Scars:

Wrinkles:

The results look quite good and as far as I know are the product of a single treatment session.

In retrospect, the use of stem cells for cosmetic purposes is quite new. On the other hand, the extensive academic peer reviewed animal model studies and these preliminary results as seen in the above photos are all quite stimulating and exciting. The problem is that I don't know of a single colleague in North America or Western Europe who performs this type of procedure. It may be because of the bureaucratic regulations set in place within these countries (sometimes an extension of religious perspectives), or simply because of traditionalist medical inertia, which I'm sorry to say is very prevalent in many of the wealthy and "advanced" countries.

To illustrate my point, I recently spoke to a plastic surgeon in Canada about this very issue. I asked if he had considered the use of stem cells to treat acne scars. He replied, "it's coming!"

I was amused so I asked him to elaborate. He then said that the use of stem cells for cosmetic purposes will likely be available in 10 or so years. I was trying my best to be diplomatic when I explained to him that this exact procedure was already being performed in Japan, Russia, Korea, Taiwan, China, etc... Needless to say he was surprised by what I had to say. It was obvious to me that although he was being polite, he was also extremely sceptical that a young Bulgarian doctor may know something he doesn't... Finally after some ensuing discussions, he told me that he felt the use of synthetic dermal fillers was "good enough" and that the results he had achieved with these substances were very positive. In essence, he was being defensive and thus tried to redeem his lack of an innovative spirit with what I call B.S.!!! On the other hand, I'm pretty sure he is now more open to learning about autologous mesenchymal injections. Let's just abbreviate this procedure as AMI from now on.

Now I want to get to the point! If you live in a city and/or country where AMI is not available, then be the one to tell the medical community about it. Be the one to tell your local dermatologist and/or plastic surgeon that the use of stem cells for cosmetic purposes is not science fiction, but rather science reality. Tell them that you are not talking about the use of embryonic stem cells, but rather about autologous adult stem cells. If need be I will give you information about how this procedure is performed since your doctor will likely not have a clue. I am here to help, and the point I am making is that you can also help yourself. Spread the information and get your local docs to start thinking about what's best for YOU the patient. Needless to say, the first doctors to start performing this procedure in your respective countries/cities will have an immense competitive advantage.

And you may just get to have the skin you've always wanted.

Best wishes and start spreading the message of change,

BRD

BRD,

I want to say a heartfelt THANK YOU for your compassion, time, and energy put into informing us the latest in Stem Cell medical technology for scar remodeling. This is really the most promising treatment for acne scaring I have heard in a very long time. You are a great treasure on this board and we are so fortunate to have you with us!

Please stay with us and keep us informed with your latest update.

I am considering the treatment at SEISHIN Regenerative Medicine Center in Japan.

http://www.saisei-iryou.com/english/cellantiaging/

But after reading about the procedure outlined on their website, I have one question.

I understand that The Celution System allows automated, precise separation and extraction of adipose tissue-derived stem cells... However, there was NO mention on their website that, after the extraction, the cells are then cultured for a period of time to increase their numbers exponentially... in order to achieve the maximum result in regeneration.

In fact, it even appears that in SEISHIN, the extracted stem cells are (or can be) injected to the scar immediately after its extraction (thus leaving no time for culturing).

In reading your introduction (your first post) on mysenchymal stem cells, I was under the impression that culturing the mysenchymal stem cells to increase its number by 1000s is an essential part of this procedure to yield maximum result in scar remodeling.

In your opinion, Is Seishin missing this important step? I would think unlikely. But why this essential step of culturing to increase numbers of mysenchymal stem cells not described?

Or maybe I am missing something here? For example, the Celution not only extracts but also cultures the mysenchymal stem cells to increase its numbers?

I can imagine your inbox must be flooded with questions, but I do hope i hear from you. THANK YOU.

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(@bulgarian-r)

Posted : 01/29/2009 10:57 pm

Hi BRD,it wasnt a recent PM but an email.

On one of the Japanese website,it says the effective period is 3 years for stem cell treatments.I thought this was permanent......some temp fillers last just as long and cost much cheaper.

Hi Tofu,

For the stem cell treatment they mention that it is "semi-permanent". What this means is that the effects are long term. In fact I consider a properly done procedure to be just as long-term as anything else within the human body... In other words, nothing as of yet escapes the degenerative forces of wear and tear brought on by the natural aging process. Either way, a 20 year old who's had this procedure performed at the highest level of competence should see the effects well into their forties. A fifty year old should likewise see the effects well into their 60s. The primary issue to consider is that the older a person, the less substantial the blood supply to dermis/hypodermis and the greater the levels of oxidation. This causes increased cellular apoptosis, which theoretically also means that any cell, including a stem cell and its lineage, will be viable for a shorter period of time.

Indeed as a person gets older, his/her tissues have less and less stem cells and this is partly also why these tissues tend to degenerate at an increasing rate. Going by that logic, I can suggest to you that increasing the amount of mesenchymal stem cells in one's dermis is a way to slow down this process, while actually even boosting the localized regenerative capacity of the organism.

The 3 year period that you ask about is actually in reference to the gingival cell therapy. A few years back a Japanese dentist invented a new therapy whereby fibroblasts from the oral mucosa are extracted, isolated, cultured and reinjected into atrophied areas of the dermis. These fibroblasts are different from normal dermal firboblasts and very appropriate for providing volumizing effects for such things as wrinkles, acne scars, etc...

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(@bulgarian-r)

Posted : 01/29/2009 11:02 pm

BRD,

Is your procedure similar to Dr. Roger Amar's "FAMI" or "AMAR" (Autologous Mesenchymal Anatomic Restoration) technique? It seems as though his procedure differs from traditional fat grafting/transfer, but I am no expert. His before and after pictures are impressive, especially in restoring large volume losses. Do you think his technique would work for superficial rolling scars?

Hi,

I don't think that the procedure I'm developing is similar to Dr. Amar's. I don't even know how Dr. Amar's procedure differes significantly from the traditional fat transfer, because I have not been able to get detailed information on his methodology. Perhaps if I ever got to speak to him I would be able to tell you more.

WORD OF CAUTION: I get the feeling that some doctors are telling their patients that they will be performing a stem cell transfer/injection, when in fact they are simply harvesting and trasnfering autologous fat. This is misleading.

My definition of stem cell injection means that the concentration of stem cells has to be increased per cubic centimetre (cc) of autologous material injected. Obviously in the traditional fat transfer, the concentration of stem cells remains the same as when taken from the area of harvesting. Worse yet, some adypocites and certainly stem cells will die before the adipose tissue is reinjected, which means the relative viable cellular mass in decreased.

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(@bulgarian-r)

Posted : 01/29/2009 11:18 pm

http://www.theamarclinic.com/index.html

Another link of Dr Roger Amar's.I was just reading the site and I find the stem cell treatment to be a better choice over silicone injection.I just hope this is not another"too good to be true" ....so many false hopes and promises from the past.

There can only be "too good to be true" if one expects this treatment to treat all types of dermal damage, scars, etc... For example, treating keloids is in my opinion more difficult. However, for people who have skin depressions, this is not just a step forward, but a leap. It can provide one with lifting volume, increased regeneration and tissue remodelling. I see nothing else that rivals this.

Nonetheless, be cautious if a doctor says he'll do stem cell injections. Make certain it's not just a traditional fat transfer. Also make sure that this person is good with their hands and adheres to the highest standards of practice, especially where live cells are being utilized for the therapy at hand. After all, improperly handled (dead) stem cells are no good...

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(@bulgarian-r)

Posted : 01/29/2009 11:31 pm

BRD,

Thank you for posting this information. I've been researching this for a while now, and I think we're on the precipice of a revolution in skin renewal.

The thing I'm curious about is how to bridge current standards of treatment with stem cell procedures. I don't think the leap will happen without a current standard "building on" a tested and accepted method.. as opposed to a new method altogether.

For example, I think a treatment that might branch out into a stem cell method is the current acellular allografts. Don't you think this might be a good delivery system for stem cell therapy? If they incorporated stem cells, this might be a breakthrough which wouldn't require starting from scratch.

--------------------------------------

Acellular allograft dermis (AlloDerm[R], LifeCell Corporation) appears to present a significant solution to treating acne scars and deep wrinkles. The material is flexible, easy to implant, has a low infection rate, and elicits no rejection response (Jones, Schwartz, & Silverstein, 1996).

The graft material has been in use since 1992 as a surface graft for burn patients, with use as an implant material more recently. Human donor skin, obtained from United States tissue banks, is processed in detergent washes to remove all the cells, eliminating targets of the rejection response.

This process yields an acellular matrix with normal human collagen structure (Livesey, Atkinson, Call, Griffey, & Nag, 1994; Livesey, Herndon, Hollyoak, Atkinson, & Nag, 1995). The processed dermis is packaged under sterile conditions, then freeze-dried with a patented process, allowing the grafts to be kept in standard refrigeration for up to 2 years after the date of initial processing and easily accessible when needed.

An additional advantage of this material is its flexibility, which allows it to be cut to fit the individual size and shape of a scar or depression. To customize the size of the graft material for the varying depth of scars, several layers can be implanted (Jones et al., 1996), or a single layer can be planed down for shallower depressions.

--------------------------------------

Hi LOVE,

I've discussed an extracellular matrix (ECM) delivery system in some of my past posts on the scarless healing thread.

You are right that utilizing something such as Alloderm in combination with mesenchymal stem cells would be quite effective. The reason is that stem cells essentially are attracted to biological scaffolds to which they can bind and proceed with differentiation.

There are some teams out there who have tried this technique on mice. The only potential problem is that there can sometimes be an immune reaction to the ECM, which would lead to inflammation and host of other cascading problems that may neutralize the beneficial effects of the injected stem cells.

The various ECM producers are constantly coming up with new products and they are getting better at removing any matter imbedded in the scaffold that may cause the problem described above. Thus even xenogeneic scaffolds such as the Acell product (powder version) may find its use as a delivery system.

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(@bulgarian-r)

Posted : 01/29/2009 11:42 pm

I'll be honest, I've been casually following this thread because the depression associated with scarring has worn on me and worn on me, so I've kind of stopped following the threads for a little bit lately.

However, I am interested in hearing what I can do to help test this procedure. Where can I get this procedure done? Where is it being developed? Who's researching it for use specifically with scars?

Bulgarian Derm., are there people that will apply this procedure as you would like to do for the right amount of money? Do you envision 100% healing?

Hi hoursafter,

I am not sure I understand your question about people doing this "for the right amount of money". Are you wondering if there are people willing to pay a relatively high price in order to have this procedure done?

As for your other question, please look at all my posts about this. I've described it in detail, especially in regard to hypotrophic (depressed) acne scars.

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(@bulgarian-r)

Posted : 01/29/2009 11:46 pm

BRD, will this stem cell treatment grow hair if the cells are injected in a depressed scar where there is supposed to be hair?

I don't know. There are teams working on this type of research, but it's too early to tell how succesful such a treatment would be for complete hair restoration. Another forumer asked me about this too and I promised to look in to it. When I do know something I'll post about it.

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(@bulgarian-r)

Posted : 01/30/2009 12:00 am

BRD,

Would you consider treating patients once you have confidence that your stem cell procedure works?

Hi again,

As I've said before, I do not solicit people on acne.org, in any way form or manner, to come to my office and receive any treatment that I offer. In fact, when people on acne.org ask to come visit me I simply refuse. It would be unethical to accept their offers in light of the circumstances at hand. You know how I feel about this particular issue from our past conversations.

The most basic reason I do things this way is that I am not on this forum to advertise myself. Thereby no one here knows my name and/or where I'm located. In other words, the help I provide herein is free of charge and I consequently don't seek public recognition or your money. In this way there are no moral hazards that could lead to unethical behaviour or the accusation of such. Thus I simply provide you (a person in need) with cutting edge information which you can then use to help yourself by finding doctors who are forward looking. Learn from this information, personalize it and present it to those who you deem qualified to hear you out.

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(@2012)

Posted : 01/30/2009 12:44 am

Summary: Some Important Points of the Isolagen Procedure offered in Beijing by Aoluosi

 

I have looked into the Chinese website (from which the before/after photos in BRD's post that started this thread). Since I read Chinese, I have summarized in brief translation the important points on the procedure below,

 

info source: http://www.aoluosi.com/detail/5563.html

 

The important point is: what is injected into your scars is your own fibroblast cultured to a large number (which, to me, seems NOT the stem cells or mesenchymal cells BRD described.) + growth factors sourced from your own blood.

 

1) The procedure is indeed Isolagen. There is an English article on Aoluosi's website:

 

http://www.aoluosi.com/detail/5546.html

 

The Chinese name used for this procedure in scar remodeling in Aolusi is eOEaeacezcc.

 

2) 10 cc of your blood + a skin sample from the back of your ear (size 0.3 cm*1 cm) is taken and cultured at China's GMP lab for 6-8 weeks, until the fibroblast content reaches the concentration at 20,000,000/ml.

 

23 different growth factors sourced from your blood, including EGF, FGF, IGF-1,PDGF-AB, TGF-beta, VEGF will also be injected to stimulate regeneration. [but I am not sure if the Growth Factors are combined with the fibroblast culture or if they are injected separately]

 

3) The above is then injected into the site of the scars. 3 injections are required and each of them 17 days apart.

 

4) A comprehensive range of cosmeticeutical topical such and supplements are prescribed (and sold to the patient, I assume) by Aolusi to be used in conjunction with the procedure.

 

5) Aolusi, the clinic in Beijin, claims to be the only one officially authorized by the Chinese government to provide the procedure in China. The director of Aolusi is Japan trained Chinese scientist/surgeon, who received his PH.d in Cosmetic (??) Surgery from Kyoto University. Aolusi claims that it has performed this procedure on thousands of patients with success.

 

6) Cost is according to the volume of the final culture you need, therefore dependent on how many scars/how large or deep the scars etc. 1 ml costs $6600 RNB = $964.884 USD = $1,185.96 CAD

 

7) [i am confused about] how long the result lasts, in some places it says 3-5 years, in other places it says 5-10 years (more for wrinkles) and there is also mentioning of semi-permanent or permanent results on their website.

 

7) You can pay an extra $235/year for Isolagen to store your cells for future use.

 

Now compare Isolagen by Aoluosi and Cellution at SEISHIN.

 

http://www.saisei-iryou.com/english/cellantiaging/

 

From my research so far, SEISHIN's procedure is closer to the stem cell therapy BRD described. because it actually uses adipose-derived stem cells. The Isolagen at Aolusi, Beijin uses fibroblast (albeit cultured to a large number), NOT stem cells.

 

However, SEISHIN does NOT culture the adipose-derived cells extracted by Cellution to a large number before injecting. SEISHIN says it is not necessary when using the Cellution. WHY? I don't know. (BRD, if you are reading this please help us on to clarify this?)

 

from SEISHIN's website:

 

"In transplantation of adipose tissue-derived stem cells with the Celutiona System, stem cells are accurately extracted from fat collected from the abdomen or thighs and injected together with injectable fat.

 

At the injection sites, stem cells produce the unique effects of angiogenesis and differentiate into adipocytes to form adipose tissue, thereby achieving very high rates of fat graft survival and providing sustained benefit. Unlike injection of fibroblasts that requires long-term culture, transplantation of adipose tissue-derived stem cells allows real-time treatment. A series of processes from liposuction to extraction and injection of stem cells are performed during a single clinical visit (about 3 hours), and re-injections are not necessary. In fibroblast injection, injectable sites are limited, and medical costs increase substantially with increasing injection sites. In our procedures, however, about 100 cc of injectable fat (adipose tissue-derived stem cells) is harvested in one fat collection session, and thus costs for one session are sufficient for injection into multiple sites."

 

At SEISHIN, Cellution procedure costs 1 million yen regardless of numbers of sites requiring injections. I will be calling SEISHIN and Aoluosi to ask many questions. If anyone has questions you would like me to ask, please post.

 

 

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MemberMember
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(@bulgarian-r)

Posted : 01/30/2009 12:51 am

icaretohelp ... is dr khan gona do the procedure that BRD is talking about ?

yes on me he said id only need platet but he will culture stem cells from my fat and combine them with platlet therapy if platet therapy isnt enough.(2 months after my PRP..im doing lipo on the same day for my nxt potential injection) ( he said they both do the same job but tthe latter which invloves liposuction would be a better option for deeper scars) he said platet therapy is also wonderfull at improving the Tone of the skin..he said some ppl with rosacea induced by a weakedn epidermis cos of acne scarring notice after PRP that there skin looks more porcelin.

if u guys have any other questions just contact him..i travvked from bahrain to london to see him..im back in bahrain now..ill be going back to the uk on the 5th and im going in for my prp and lipo on the 6th..the lipo is for my next injection which would be PRP and Stem cells from the fat.

 

Hey icaretohelp,

How's it going?

I was quite certain that you were not going to be getting the same treatment on the 6th of this month as I have presented in this thread. This has to be clarified, because many people still incorrectly believe that you will be updating them on stem cell injections in a week.

Thus I must clearly explain to everyone that what you're getting done on the 6th is in no way, form or fashion directly related to mesenchymal stem cell injections. Platelet Enriched Plasma (PRP) therapy (what you'll be getting) is also called autologous blood injections... Please post about this in the autologous blood injection thread since it's more appropriately suited to that topic and many people will be interested to know your experience. Believe it or not, most dermatologists have not heard of blood injections and thereby most people on acne.org did not know what I was talking about when I posted about it in June of 2008.

Now then, while blood contains stem cells, it is a very small amount and thus not the primary histological mechanism by which tissue regeneration occurs. I have discussed this in detail in the blood injection thread and I have been performing this treatment for a few years now. Furthermore the stem cells present in blood are called peripheral blood stem cells (PBSC) and are generally hematopoetic, not mesenchymal. Their function is different then the type of stem cells I discuss herein.

Anyway, I like to do blood injections because they are simple, inexpensive and very safe. They are also quite effective.

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