Problem is it's not based on science, at least not the health science. And your "science" is just supposition. You never focus on the whole body, but look at isolated temporary cause to effect things trying to find something you want to alter externaly. Did your scientific article if there is one, about accutane provoking telomere shortening, was done before and after treatment? and month after the end? and even if it was the case, and there was a telomere shortening, in multiple part of the body, even there, it would only show a symptom, not a cause.

What are you talking about with your larger plan, you throw your false claim about telomere and i can't say it's stupid? i even told you that according to science, telomere can shorten and then get longer with time and environmental factors. You so desesperate to find a magic solution, to hold on an external magic solution, that you refuse to understand that the body is a living thing working as a whole, that deals itself with it's homeostasis. You want to cure the magic solution for acne (accutane), with an other, with the same ideology, an external solution. It won't work. Nothing external can bring the perfect homeostasis required for the body to work idealy, everything is connected and affect the rest inside the body, you juste have to look at what accutane did to understand it.

9 hours ago, blackfox01 said:

I suffer from PFS so i hope you dont mind me being here. I have literally talked to HUNDREDS of people across the globe who have been fucked by finasteride, Cipro, SSRIs, accutane etc

The similarities are mind boggling. One of the interesting one is how people take cipro many times with zero problems, or they take accutane many times with no problems. THen they take it one last time and BAM they get fucked, and 10-15 years later they are still screwed, but feel abit better mentally due to neuro-adaptation.

This tells me we need a differnt approach at trying to find the cause. If Accutane or Finasterides effect on Androgens was so terrible as we say after cherry picking many studies, why did lots of us feel FINE whilst on them?? Some people took Finasteride for 10 fucking years with ZERO side effects. Then they quit taking it cold turkey and 2 weeks later BAM something was screwed up.

From the androgenic angle, my theory is that these drugs cause a receptor to become sensitive then when they are withdrawn the receptor gets bombarded and the body shuts down the pathway as a protective mechanism. The second the pathway is turned off there are thousands if not trillions of downstream effects depending on ones individual biochemstiry which leads to a constant viscous cycle and they body can never recalbrate the pathways.

It seems some people can recover slowly over the next 1-5 years, whereas others literally stay the same for 15+. I know a guy who crashed on accutane 15 years ago and hrs no better.

From the recovery stories, it seems you either find a natural or synthetic product that interacts on the exact layer thats broken, and you megadose it and flick some switch which causes a recovery, or you go the hollistic route and acquire perfect health whilst in this terrible neuro-immune-endocrine state and allow the body to heal, which is borderline impossible.

In the meantime, i have started chelating mercury with the cutler protocal, and in the future i might try an epigenetic agent like EGCG in a megadose. A PFS guy who was also a cancer researcher managed to recover by megadosing a natural HDAC inhibitor once a month. He had PFS for 10 years before doing it.

Dont get me wrong though, the symptoms of these drug induced syndromes are similar but have their own individual characteristics depending on the drug. But i think the mechanism of action is the same.

I've been suffering from the side effects from accutane for about 6 years now. Some things have resulted in some improvements however;

- Tribulus - A 1gram capsule gave me really bad brain fog for about 2 days, but then I had much improved sexual sides for the following 3-4 days, but didn't last when I continued taking it.

- RSO/Vaping weed - some improvement to sexual sides, but very inconsistent - possibly just due to increased levels of dopamine.

- Pine pollen - improved sexual sides and mental clarity for about 3-4 weeks, until I got symptoms of high estrogen and stopped

- Reservatrol - slight improvement in sexual sides, but also headaches so stopped

- Zero fat diet and/or calories restriction? - Recent issue with a bilary duct obstruction meaning I felt horrendous about 1 hour after eating, especially worse with fatty foods where bile is released. Lasted almost a week, and I was eating only a little and learnt to avoid anything with fat in as this would cause a release of bile and made me feel terrible. Resulted in 3-4 days of much improved sexual sides after issues went away (intending to try a week of zero/low fat food and see if I can recreate the benefits)

Hey tanedout- maybe you should try liver flushing and coffee enemas. Sounds like the reason you get more side effects when eating fats is because bile is released(which may have accutane in it) but your body reabsorbes a lot of your bile again. Coffee enemas and liver flushes help to excrete the toxic bile.

10 hours ago, anonyy said:

If a drug cause a defect in the homeostasis of the body, the solution is not to take more drugs, it's to let the body restore it by itself, because only he can. You think of your body as a dead thing that needs to be artificially influenced on a perticular isolated aspect (here telomere), there is nothing more ignorant than that.

Homeostasis after Accutane - when's that going to kick in???

I'm 18 years past being poisoned and if anything I'm getting worse with all my side effects. I'm open minded about new medicine and supplements!!

Doesn't mean at the same time you can't do liver flushes, clean up diet, make positive lifestyle changes etc. You can take a new supplement or medicine and still do all the other things you believe are beneficial to the body!!??

1 hour ago, Chris16 said:

Hey tanedout- maybe you should try liver flushing and coffee enemas. Sounds like the reason you get more side effects when eating fats is because bile is released(which may have accutane in it) but your body reabsorbes a lot of your bile again. Coffee enemas and liver flushes help to excrete the toxic bile.

Yeah I've tried one liver flush and got nothing from it, but I'll probably try a few more anyway, and may look into coffee enemas too. I know some people have said they've benefited, but I don't expect anything groundbreaking really!

You know what's #$%^ up about this horrible drug most people get the WORST side effects years / decades later as I did but they hand it out like candy to teenagers and young adults usual for mild acne , in reality this drug shouldONLY be used for severe cystic nodular acne even then life might be better without being poisoned for life me thinks.... does it really save more lives than it takes hmmm.... 1/3 of derms won't even use it in there practice that says something about the nature of this chemotherapy drug they know it's a horrible poison NOT a miracle drug those don't exist .

Among other things isotretinoin directly affects the brain causing a roughly 21 percent decrease in frontal orbital brain activity FACT proven by MRI's and countless LAWSUITS , what this means is that person will live the rest of his / her life with permanent brain damage again FACT. And that's just 1 of the side effects .

1 hour ago, TrueJustice said:

Homeostasis after Accutane - when's that going to kick in???

I'm 18 years past being poisoned and if anything I'm getting worse with all my side effects. I'm open minded about new medicine and supplements!!

Doesn't mean at the same time you can't do liver flushes, clean up diet, make positive lifestyle changes etc. You can take a new supplement or medicine and still do all the other things you believe are beneficial to the body!!??

Yes you can take useful supplement, like herbs, or even vitamin D or A (whole foods better), but main thing to let homeostasis kick in it's to permit the body do it, so it means detox and raw food diet (mainly fruits), it's pretty hard to do but it's the only way.

A friend who already took accutane back then went to the dermatologist for some acne, it was very little but.. they straight wanted to give him accutane again. So you are right yes, they give it like it was nothing. My own dermatologist told me it was something "strong", that i needed to be sure i wanted it, but never mentioned ofc what it can really do. So i believe they know it's dangerous, but still give it to people...

https://www.lawyersandsettlements.com/lawsuit/accutane.html?opt=b&utm_expid=3607522-13.Y4u1ixZNSt6o8v_5N8VGVA.1&utm_referrer=https%3A%2F%2Fwww.google.ca%2F

It's been removed from the market in the usa? because in europe it's supposed to be removed too but still sold as generic.

1 hour ago, anonyy said:

It's been removed from the market in the usa? because in europe it's supposed to be removed too but still sold as generic.

You and I know why it was removed but what reason did they give for removing from the market??

I'm keen to understand what they've admitted to in relation to adverse side effects?

I'd love to give the makers a taste of their own medicine - get them to take roaccutane and see how they fare afterwards!!!

Anonyy, I usually try to see the best in people, but your reply to me was full of blatently false information. Allow me to clear things up in the most polite way possible.

6 hours ago, anonyy said:

Problem is it's not based on science, at least not the health science. And your "science" is just supposition.

I said Accutane causes cellular death. Scientific proof:

"We have to appreciate that isotretinoin does not exclusively targets apoptosis of the sebaceous glands as proapoptotic drug effects have been observed in several unrelated cell systems and explain all adverse effects of isotretinoin and other retinoids. . . .exerts apoptotic effects in multiple cell types like the muscle, the bone and the brain."

I said Accutane metabolizes into a drug that causes telomere shortening. Here is a paper explaining it. Yes, telomeres may shorten and expand at times, but Accutane DISRUPTS this process via the telomerase enzyme. Lastly, I linked to the papers showing how there is a compound capable of upregulating telomerase. I have not seen you link any papers to support/refute any claims. Where, specifically, have I been unscientific? Let's argue the logic. I could be wrong; perhaps the compound I discussed will have no effect on my symptoms! However, you need to explain HOW my logic is off before I can accept what you are saying.

6 hours ago, anonyy said:

hold on an external magic solution, that you refuse to understand that the body is a living thing working as a whole, that deals itself with it's homeostasis.

It is with this quote that I cannot help but believe you are being malicious. (And I do so reluctantly, because I like to see the best in people.) Please stop intentionally misrepresenting my point of view. It degrades the quality of discussion. Just because I talk about one compound rejuvenating cellular function does not mean I want everyone to ignore other facets of health. That is absolutely ridiculous. When did I call Epitalon magical? I specifically said I wasn't getting my hopes too high. If anything, there is no better time to address ALL of your medical concerns by living healthy than when your cells are functioning optimally. And that is EXACTLY what Epitalon, the natural substance found in the human body, is supposed to do. It would likely work best in conjunction with a healthy diet and exercise. When you talk about your adrenal supplements, I don't accuse you of not realizing "the body functions as a whole." We are allowed to talk about one supplement at a time without being accused of losing focus.

6 hours ago, anonyy said:

Nothing external can bring the perfect homeostasis required for the body to work idealy

Well, that's awkward. Food is external. And so are those vitamin D supplements you talk about. In fact, two of the three people who liked the post you made where you called me "ignorant," talked in support of external supplements as well. (Oh, the irony.) Every food item or supplement is a complex composition of chemicals. I believe in discussing all options; not shaming people. I believe we CAN talk about components of health without "losing sight of the bigger picture." I extend this courtesy to those who disagree with me, but I WILL call out inconsistencies when I see them.

2 hours ago, Gladiatoro said:

https://www.lawyersandsettlements.com/lawsuit/accutane.html?opt=b&utm_expid=3607522-13.Y4u1ixZNSt6o8v_5N8VGVA.1&utm_referrer=https%3A%2F%2Fwww.google.ca%2F

The lawsuits against Accutane are the best thing that have ever happened. You have to hit a corporation where it hurts (their profits), or they'll never let up. A recent 2009 settlement demonstrated that Roche stifled internal research that showed a CASUAL relationship between Accutane and IBD. The studies found Accutane erodes the intestinal tract. Now, whenever a medical professional or random internet commenter tries to argue that the link between Accutane and gastrointestinal diseases isn't "proven," I can point them to the court settlements. They revealed invaluable information to the public. However, Roche still gets away with a lot because the court documents are permanently sealed, so we'll never be able to see the actual studies in their entirety. It would have been much bigger news if everything was released, but that's the power of attorneys!

6 hours ago, tanedout said:

I've been suffering from the side effects from accutane for about 6 years now. Some things have resulted in some improvements however;

- Tribulus - A 1gram capsule gave me really bad brain fog for about 2 days, but then I had much improved sexual sides for the following 3-4 days, but didn't last when I continued taking it.

- RSO/Vaping weed - some improvement to sexual sides, but very inconsistent - possibly just due to increased levels of dopamine.

- Pine pollen - improved sexual sides and mental clarity for about 3-4 weeks, until I got symptoms of high estrogen and stopped

- Reservatrol - slight improvement in sexual sides, but also headaches so stopped

- Zero fat diet and/or calories restriction? - Recent issue with a bilary duct obstruction meaning I felt horrendous about 1 hour after eating, especially worse with fatty foods where bile is released. Lasted almost a week, and I was eating only a little and learnt to avoid anything with fat in as this would cause a release of bile and made me feel terrible. Resulted in 3-4 days of much improved sexual sides after issues went away (intending to try a week of zero/low fat food and see if I can recreate the benefits)

MEDIHERB tribulus cured some PFS guys. But they took 12 capsules a day (800mg+ protodiscion) And it crashed their bodies but when they quit they got a better baseline

Email from a prominent PFS doctor:

Any thoughts?

" Please stop intentionally misrepresenting my point of view "

that's what you do, when you talk about external about foods & herbs, who truly helps the body to get back to homeostasis from the inside, whenyou apparently advocateexternal chemicalssince you look at something to alter back artificially about your telomerase, that's not the same thing. but of course, the best way to get back the balance, is fasting, herbs are just an help and are natural and made for us by mother nature, notyour synthetic supplements.

"When you talk about your adrenal supplements, I don't accuse you of not realizing "the body functions as a whole."

Because it's not the same thing, i focus on organs and glands regeneration, with herbs mainly, vitamin D & A you can take it from foods, so it's not an issue. You focus on theories about a bit of dna, and a symptom only, it's not the same thing again.

I told you in every post why your logic is wrong, accutane affect everything inside the body, if it affect telomerase, it's just 1/9999999 of what it affectat the microscopic level, you can't artificially alter them all back with synthetic drugs (only logical way to do it according to the path you follow looking at isolated things and symptoms), only the body itself canbring back homeostasis.

And even if your telomerase supplement can treat the symptom only, what else it will affect? like accutane, it will destroy even more homeostasis of the whole body, affecting in cascade all the rest, since everything inside the body is working as a whole. And like digestive enzymes, or testosterone for exemple, if you take it it will disturb homeostasisbut also your body will become dependent on it, and will stop working by itself to compensate. And it will take even more time to bring everything back into balance.

@anonyy
Accuity_Drane provides valuable insight and information. You might not like it or agree with it, but I'm sure some people will find his addition to this whole forum interesting. I for one will take any bit of information and take it into account. I'm not rejecting any theory, purely because nobody knows for sure what the problem is. If we knew, we'd have solved it by now. If you could kindly refrain from using this forum as a show of your ego, i'm sure it would be beneficial for all. There's not point acting like childs in a playground, we're all in the same situation and we're all trying to help each other here. I understand you hold firmly to your beliefs, and you've already made it clear in your many previous posts. There's no need to repeat yourself.

-

In the meantime, I've received my DNA results and will get my complete Blood Work next week. I've confirmed I don't have MTHFR issues, which is good, though I do have many other gene variations. I'm currently learning about the genes and their impact. I'm not sure how significant the impact of having different genes affect our reaction to Isotretinoin, but I figure those that were strongly impacted could have some similarities in their genetic code.

Of interest is I've unknowingly been ingesting the animal version of Vit A though the Omega 3 supplements I was taking. I've discontinued its use and I think I've been doing better since.

Taurine usage seems beneficial, but I've learned through the below results (CBS gene) that I don't tolerate sulfur well, and taurine is one.. so, we'll see. Interestingly, I've had moments the days after taking ~3g taurine where I was suddenly tearing up. Not sad, just tears flowing out. It never happened before. My eyes are usually much too dry and its helped a lot.

Here are my Methylation results:

I have ONE clear detox issue in my DNA, as follows:

I found it interesting when it said epitalon was secreted by the pituitary. My current hypothesis is that isotretinoin and its toxicity potential, wasn't able to discern from one exocrine gland from another. I believe it taxed all of our glands, endocrine included. The glands regulate so much, and it would explain why we get a clean bill of health from just blood tests. Isotretinoins ability to affect glands and histamine receptors would definitely have an effect on serotonin too, which is mind boggling why they won't acknowledge the depression in some individuals.

As as far as supplementation goes, there are good ones you can take for general well being (coq10, c, d, ginseng, etc.), scientifically backed, obviously. But, as far as supplementation curing anything, unless you have a lab result deficiency, is a bit far fetched. I will say I have seen definitive results using digestive enzymes and creating healthy brown stools after dealing with pancreatitis and light yellow stools on a consistent basis. It's no secret why doctors recommend enzymes for those who need digestive support.

anyways, I like how this page started out. I hope we can reverse the damage done, if it is indeed apoptosis, we have an uphill battle as we are not scientists, and we will have to work together.

" Accuity_Drane provides valuable insight and information. "

He provide information everyone already know, that speculation about telomere date from 10 years ago. But obviously it never helped anyone, it's just one of the multiple theories from people who look at an other drug to alter artificialy their body. You won't cure accutane induced issues, with an other drug, or focusing on symptoms, or trying to affect only a specific isolated aspect of the body, here a bit of dna, that's allopatic thinking.

On 6/28/2016 at 6:58 AM, tryingtohelp2014 said:

new results

After 2 months of 4000iu Vitamin D... my levels have increased from 9 to 36.9

after 2 months of  copper, im still in the low normal range. (i believe this shows how much we really need to improve body stores.)

i also tested my serum Vitamin A levels... normal.   

Zinc Normal (after taking copper only.... again, copper alone doesnt deplete zinc... zinc on the otherhand does deplete copper.  my zinc actually went up since my last test.)

I will get one more ceruloplasmin test in 5 days, and i will up my copper from 2mg to 4mg for the 5 days before the test.   i would be willing to bet my ceruloplasmin goes alot higher, while reducing any "toxic" copper lol.   If that happens, it will confirm a true copper deficiency in the liver.

 such a joke on this thread about toxic copper.

April test                            .June test          

ceruloplasmin 17.3  .............18.9 
copper       ..........75..................78
zn       ..................76..................83           

i want to get a biotin test.  13 cis retinoic acid messes up the biotinidase enzyme.... many many people report more oily skin taking biotin.   some suggest thats how accutane works, just like B5 does... by disrupting biotin.
http://www.ncbi.nlm.nih.gov/pubmed/10325581
 

When do you get these results back? I am curious to hear about them because I recently experienced serious benefits in regards to exercise and energy from copper supplementation (not as much as guitarman did, just 2mg a day of Pure Encapsulations Copper Glycinate).

I know some people are really hating on copper supplementation right now and possibly for very good reason, but we have all been affected differently. I understand everyone wants to help find the right solution but I would appreciate it if people would please refrain from shit talking this post. If you have something to add I will be much more likely to listen if you don't make me look or feel like an idiot.

@Kynarr

Since you appear to understand what means those results, can you tell me what means mine according to this mthfr theory? (didn't do the test for that reason, just to precise):

looks like i have one on three of the mthfr alleged problem, didn't seems to have prevented me to heal most of my issues.

6 hours ago, Kynarr said:

Taurine usage seems beneficial, but I've learned through the below results (CBS gene) that I don't tolerate sulfur well, and taurine is one

That is my problem as well. I have some mutations in my CBS genes that supposedly mean I should avoid sulfur products, yet so many sulfur supplements are regarded as useful. I also have a gene that implies I should be taking the bio-available form of folate. So I've been doing the methyl-folate and methyl-b12 protocol for two months now. I have been been having more good days (cognitively and mood wise) lately, but I have no idea what to attribute it to.  Interestingly, I also have the Apoe-e4 gene, which is associated with an increased Alzheimer's risk. To me, this is significant because studies show that those people who go through chemotherapy and possess the Alzheimer's gene (Apoe-e4) are MORE likely to show cognitive decline and memory issues post-chemotherapy than those who do not have the gene. It's just yet another angle to look into, as many of us feel like we have chemo-brain. Ah! As if we don't have enough to look into already!
 

5 hours ago, macleod said:

healthy brown stools after dealing with pancreatitis and light yellow stools on a consistent basis. It's no secret why doctors recommend enzymes for those who need digestive support.

anyways, I like how this thread started out. I hope we can reverse the damage done, if it is indeed apoptosis, we have an uphill battle as we are not scientists, and we will have to work together.

Yes, although Accutane is confirmed to cause apoptosis, the question remains, is it the source of our problems? What about everyone's theories that Accutane affects DHT just as Propecia does, and that it acts as a 5-alpha-reductase inhibitor? I only recently started looking at this angle and it's ridiculous how much many of us have in common with those who took Propecia. I don't know where to go from here, but I try to throw out ideas where I can.

I will have to look into the enzymes. So far, home-made Kefir has been an absolute game-changer in dealing with my IBS symptoms. All thanks to a random recommendation I read 100 pages back!
 

13 hours ago, anonyy said:

 (only logical way to do it according to the path you follow looking at isolated things and symptoms),

5 hours ago, anonyy said:

You won't cure accutane induced issues, with an other drug, or focusing on symptoms, or trying to affect only a specific isolated aspect of the body, here a bit of dna, that's allopatic thinking.

With all due respect, I am done replying to you due to your continued misrepresentation of my viewpoints. I know it is garnering likes, but it is imperative that we accurately portray other peoples' ideas. Epitalon does not affect an isolated aspect of the body. It affects multiple TYPES of cells and multiple systems in the body. Also, I never claimed it was the end-all cure. I believe supplements are an aid, just like you do (but our methods obviously differ). Certainly other aspects of health should be considered. (What good are healthy cells with lengthy telomeres if your diet is off? Of course I acknowledge the big picture matters.  )

48 minutes ago, ACCUiTy_drANE said:

That is my problem as well. I have some mutations in my CBS genes that supposedly mean I should avoid sulfur products, yet so many sulfur supplements are regarded as useful. I also have a gene that implies I should be taking the bio-available form of folate. So I've been doing the methyl-folate and methyl-b12 protocol for two months now. I have been been having more good days (cognitively and mood wise) lately, but I have no idea what to attribute it to.  Interestingly, I also have the Apoe-e4 gene, which is associated with an increased Alzheimer's risk. To me, this is significant because studies show that those people who go through chemotherapy and possess the Alzheimer's gene (Apoe-e4) are MORE likely to show cognitive decline and memory issues post-chemotherapy than those who do not have the gene. It's just yet another angle to look into, as many of us feel like we have chemo-brain. Ah! As if we don't have enough to look into already!
 
Yes, although Accutane is confirmed to cause apoptosis, the question remains, is it the source of our problems? What about everyone's theories that Accutane affects DHT just as Propecia does, and that it acts as a 5-alpha-reductase inhibitor? I only recently started looking at this angle and it's ridiculous how much many of us have in common with those who took Propecia. I don't know where to go from here, but I try to throw out ideas where I can.

I will have to look into the enzymes. So far, home-made Kefir has been an absolute game-changer in dealing with my IBS symptoms. All thanks to a random recommendation I read 100 pages back!
 
With all due respect, I am done replying to you due to your continued misrepresentation of my viewpoints. I know it is garnering likes, but it is imperative that we accurately portray other peoples' ideas. Epitalon does not affect an isolated aspect of the body. It effects multiple TYPES of cells and multiple systems in the body. Also, I never claimed it was the end-all cure. I believe supplements are an aid, just like you do (but our methods obviously differ). Certainly other aspects of health should be considered. (What good are healthy cells with lengthy telomeres if your diet is off? Of course I acknowledge the big picture matters.  )

Further to your point - what good are lengthy telomeres, and a clean diet if your Liver/Gallbladder is not functioning properly??

Again those who claim to be cured did so through multiple liver flushes ( some upwards of 15 or more I believe )

The question for me is - has anyone completed around this many and had NO positive result??

Starting my 5th cleanse next week

SOD gene therapy for ED

The reduced NO bioavailability contributing to age-related ED can also be caused by the enhanced inactivation of NO due to the oxidative stress in the aging penis.^40,41,42,43Although NO is rapidly diffusible from nerves or endothelial cells to the neighboring smooth muscle cells for the induction of smooth muscle relaxation, it can be scavenged by its interaction with superoxide anion (O₂^-) within vessel walls or corporal sinusoids to form the toxic molecule peroxynitrite (ONOO^-).^44,45O₂^-is involved in oxidative stress and increased O₂^-production in aging may contribute to vascular or smooth muscle dysfunction observed in the normal aging process through its destruction of NO.⁴⁶Superoxide dismutase (SOD), an antioxidant enzyme catalyzing the conversion of O₂^-to H₂O₂and O₂, plays an important role in the protection of cells against O₂^-radicals and the prevention of the formation of peroxynitrite, which is extremely cytotoxic and contributes to tissue injury, vascular tone alteration, and organ dysfunction.⁴⁷There are three known isoforms of SOD and the extracellular superoxide dismutase (EC-SOD) is thought to play a critical role in modulating the redox state of the vascular interstitium and thereby prevents the pathophysiological effects of O₂^-in the vasculature.^45,48EC-SOD is released from cells into extracellular space such as blood, lymph, synovial fluids, and cerebrospinal fluid, highly expressed in blood vessels, and is the primary extracellular antioxidant enzyme for O₂^-.^48,49,50The increased levels of O₂^-in the endothelium and smooth muscle of aging corpora cavernosa may cause the decrease in NO bioavailability observed in the aging penis. Thus reducing local O₂^-level by intracavernosal EC-SOD gene transfer may be an effective method to preserve NO bioavailability or bioactivity in the penile vasculature. Therefore, the following experiments were carried out to: (1) investigate the expression level of O₂^-in the penis of young and aged rats; (2) examine the effect of adenoviral gene transfer of EC-SOD to the penis to determine the consequence of overexpression of EC-SOD on O₂^-level and erectile function in the aged rat. In the cavernosal tissue of aged rats, lucigenin-enhanced chemiluminescence assay showed a three-fold increase in superoxide formation, and the oxidative fluorescent probe hydroethidine analysis indicated higher superoxide levels throughout the aged penis. This increase in superoxide level was associated with the impaired cavernosal nerve-mediated and agonist-induced erectile responses, increased nitrotyrosine staining, and decreased cGMP level. However, there was no compensatory change in cavernosal EC-SOD mRNA or protein in the aged rat. Intracavernosal injection with AdCMVEC-SOD (an adenoviral vector containing the EC-SOD under the control of CMV promoter) into aged rats resulted in a significant increase in erectile responses to cavernosal nerve stimulation, acetylcholine, and zaprinast to a magnitude similar to young rats.In vivoadenoviral gene transfer of EC-SOD to the penis resulted in higher expression of EC-SOD mRNA and protein, higher SOD activity and cGMP level, and lower nitrotyrosine staining. These data provide evidence in support of the hypothesis that ED associated with aging is related in part to an increase in cavernosal superoxide formation. Intracavernosal EC-SOD gene transfer reduces superoxide formation, restores age-associated erectile function and may represent a novel therapeutic strategy for the treatment of ED.⁴²

Oxidative DNA Damage by Vitamin A and Its Derivative via Superoxide Generation

Recent intervention studies revealed that -carotene supplement to smokers resulted in a higher incidence of lung cancer. However, the causal mechanisms remain to be clarified. We reported here that vitamin A (retinol) and its derivative (retinal) caused cellular DNA cleavage detected by pulsed field gel electrophoresis. Retinol and retinal significantly induced 8-oxo-7,8-dihydro-2-deoxyguanosine formation in HL-60 cells but not in H₂O₂-resistant HP100 cells, suggesting the involvement of H₂O₂in cellular DNA damage. Experiments using³²P-labeled isolated DNA demonstrated that retinol and retinal caused Cu(II)-mediated DNA damage, which was inhibited by catalase. UV-visible spectroscopic and electron spin resonance-trapping studies revealed the generation of superoxide and carbon-centered radicals, respectively. The superoxide generation during autoxidation of retinoids was significantly correlated with the formation of 8-oxo-7,8-dihydro-2-deoxyguanosine, although the yield of carbon-centered radicals was not necessarily related to the intensity of DNA damage. These findings suggest that superoxide generated by autoxidation of retinoids was dismutated to H₂O₂, which was responsible for DNA damage in the presence of endogenous metals.

vitamin A is a good acceptor and donor of electrons in chemical reactions, its properties appear to be very carefully protected by retinol-binding proteins and other endogenous antioxidantsin vivo(24). However, pharmacological amounts of the supplements above physiological amounts may perturb key physiological processes.

51 minutes ago, TrueJustice said:

Further to your point - what good are lengthy telomeres, and a clean diet if your Liver/Gallbladder is not functioning properly??

Again those who claim to be cured did so through multiple liver flushes ( some upwards of 15 or more I believe )

The question for me is - has anyone completed around this many and had NO positive result??

Starting my 5th cleanse next week

Yes, thank you for taking the time to understand my point. I respect your technique and am open to trying it. Can you link me a source that explains how to properly conduct a liver flush? The simpler it is explained, the better. The mental fog is real.

Protection by carnosine-related dipeptides against hydrogen peroxide-mediated ceruloplasmin modification.