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4 hours ago, Colinboko said:8 hours ago, macleod said:We're all allowed to vent once and a while, especially if you are a veteran member.Vent elsewhere. This should be a thread that shines light on a dark situation and gives people strength to keep fighting.
This thread should and has been a lot of things the past 8 years I've been here. Ultimately, veteran members should get a little bit more leeway in my opinion, as long as they stay within the websites rules and guidelines.
Just took L-Tyrosine, and Gaba for the first time in months. Made me realize that my mental health has only gotten worse. Gotta love it... Atleast my libido increased to maybe 1/5 of what it used to be, but maybe its just spring.
anyway, what would be the most important food to avoid as opposed to the most important to have?
Been posted before. But it would be nice to test this once and for all. If not on a DNA level, I know there was some expensive and elaborate blood tests that Rich talked about.
Vitamin A and its derivatives induce hepatic glycine N-methyltransferase and hypomethylation of DNA in rats.
Abstract
Regulation of S-adenosylmethionine (SAM) and the SAM/S-adenosylhomocysteine (SAH) ratio by the key cytosolic enzyme glycine N-methyltransferase (GNMT) is essential in optimizing methyl group supply and subsequent functioning of methyltransferase enzymes. Therefore, inappropriate activation of GNMT may lead to the loss of methyl groups vital for many SAM-dependent transmethylation reactions. Previously, we demonstrated that the retinoid derivatives 13-cis- (CRA) and all-trans-retinoic acid (ATRA) mediated both the activity of GNMT and its abundance. The present study was conducted to determine whether vitamin A had a similar ability to up-regulate GNMT and to assess the biological importance of GNMT modulation by examining both the transmethylation and transsulfuration pathways after retinoid treatment. Rats were fed a control (10% casein + 0.3% L-methionine) diet and orally given retinyl palmitate (RP), CRA, ATRA or vehicle daily for 10 d. RP, CRA and ATRA elevated hepatic GNMT activity 32, 74 and 124%, respectively, compared with the control group. Moreover, the retinoid-mediated changes in GNMT activity were reflected in GNMT abundance (38, 89 and 107% increases for RP-, CRA-, and ATRA-treated rats, respectively). In addition, hepatic DNA, a substrate for SAM-dependent transmethylation, was hypomethylated (approximately 100%) after ATRA treatment compared with the control group. In contrast, the transsulfuration product glutathione was unaffected by retinoid treatment. These results provide evidence of the following: 1) vitamin A, like its retinoic acid derivatives, can induce enzymatically active GNMT; and 2) inappropriate induction of GNMT can lead to a biologically important loss of methyl groups and the subsequent impairment of essential transmethylation processes.
if nobody has gotten this sort of test in the past, this would be a good test to take when it comes to process of elimination.
http://www.mayomedicallaboratories.com/test-catalog/Clinical+and+Interpretive/9265
Amino Acids, Quantitative, Plasma
LIS Resources
- Setup Information:
Excel | PDF - Sample Report
- Abnormal Report
- Setup Reference Guide
Useful For
Evaluating patients with possible inborn errors of metabolism
May aid in evaluation of endocrine disorders, liver diseases, muscle diseases, neoplastic diseases, neurological disorders, nutritional disturbances, renal failure, and burns
Testing Algorithm
Includes quantitation of the following amino acids: taurine, threonine, serine, asparagine, glutamic acid, glutamine, proline, alanine, citrulline, alpha-amino-n-butyric acid, valine, cystine, methionine, isoleucine, leucine, tyrosine, phenylalanine, beta-alanine, ornithine, lysine, histidine, argininosuccinic acid, allo-isoleucine, arginine, phosphoserine, phosphoethanolamine, hydroxyproline, glycine, aspartic acid, ethanolamine, sarcosine, 1-methylhistidine, 3-methylhistidine, carnosine, anserine, homocitruline, alpha-aminoadipic acid, gamma-amino-n-butyric acid, beta-aminoisobutyric acid, hydroxylysine, cystathionine, and tryptophan.
Clinical Information
Amino acids are the basic structural units that comprise proteins and are found throughout the body. Many inborn errors of amino acid metabolism, including phenylketonuria and tyrosinemia, have been identified. Amino acid disorders can manifest at any age, but most become evident in infancy or early childhood. These disorders result in the accumulation or the deficiency of 1 or more amino acids in biological fluids, which leads to the clinical signs and symptoms of the particular amino acid disorder.
The clinical presentation is dependent upon the specific amino acid disorder. In general, affected patients may experience failure to thrive, neurologic symptoms, digestive problems, dermatologic findings, and physical and cognitive delays. If not diagnosed and treated promptly, amino acid disorders can result in mental retardation and death.
Treatment for amino acid disorders includes very specific dietary modifications. Nonessential amino acids are synthesized by the body, while essential amino acids are not and must be obtained through an individual's diet. Therapeutic diets are coordinated and closely monitored by a dietician and/or physician. They are structured to provide the necessary balance of amino acids with particular attention to essential amino acids and those that accumulate in a particular disorder. Patients must pay close attention to the protein content in their diet and generally need to supplement with medical formulas and foods. Dietary compliance is monitored by periodic analysis of plasma amino acids.
On a different note. If you want to find out if accutane is still floating around in your blood, you find a university that can replicate this study.
Determination of 13-cis-retinoic acid and its major metabolite, 4-oxo-13-cis-retinoic acid, in human blood by reversed-phase high-performance liquid chromatography.
Abstract
A high-performance liquid chromatography (HPLC) method for the quantitation of 13-cis-retinoic acid (13-cis-RA) and its major metabolite, 4-oxo-13-cis-RA, in human blood has been developed. The method includes extraction of 1 ml of blood with diethyl ether at pH 6 and the analysis of the extract by reversed-phase HPLC with solvent programming and detection at 365 nm. The quantitation ranges for 13-cis-RA and 4-oxo-13-cis-RA are 10--2000 and 50--2000 ng/ml of blood, respectively. The method also provides estimates of the concentrations of all-trans-RA and 4-oxo-all-trans-RA. The mean intra- and inter-assay variabilities for all four compounds were 6% or less. The method separates 13-cis-RA and 4-oxo-13-cis-RA from 9-cis-RA, all-trans-RA, 4-oxo-all-trans-RA, and some other possible metabolites, such as hydroxy and epoxy retinoic acids. The method has been successfully applied to the analyses of over 1200 blood samples from four 13-cis-RA clinical studies.
On 4/10/2017 at 2:06 AM, hatetane said:Time to be proactive!!
There is going to be a review of accutane in JUNE this year.
I want each and everyone of you from Europe to write the best email you can.
Send it to the representatives of your country and to as many independents as possible.
For those of you outside of the UK please send to all independents and to as many others as you can especially
to the MHRA in the UK.
This as a minimum, everyone send to as many representatives as you can. Once you have composed an email it is simply a matter of re-sending.
You must include your name ( data protection will protect you) age, dosage, duration and how long post A.
A complete and thorough list of all your side effects.
You must tell your story about how and when your sides evolved.
Write about your full experience but I especially ask that you concentrate on sexual dysfunction (brain - penis disconnect) and any sides that are not listed on the PIL.
For anyone who has or still is - talk about depression and try and describe it. I believe that many doctors think you are all depressed because of your acne and you really have to convey how this unique depression gets hold of you and how long it lasts.
For those of you who feel they have had there personalties change and or depersonalisation please describe it.
Most importantly talk about any suicidal ideation or attempts.
Please mention how accutane has effected and or interrupted your study/work.
Mention how you feel about accutane and any regrets you might have about taking it.
Write about any recovery and the time this has taken.
Add a brief detail about the response of you doctors and finish of with:
1 I have reported my sides to .......
2 I have never reported my side effects.This is your opportunity to protest and have your voice heard. It will probably take you an hour which is less time than it would take to visit you GP.
I want you all to post on here when you have done it so as to encourage others to participate.
This is not a lot to ask, no excuses are acceptable .
We are trying to get better 'Patient Information' for anyone being giving accutane in the future - if you feel stronger than this then say so.Please, Please participate.
http://www.ema.europa.eu/ema/index.jsp?curl=pages/contacts/PRAC/people_listing_000112.jsp#MNMSPlease copy and paste on any other forums you use and let us know that you have done this.
Many many thanks.
Who is participating - PM me please
On 4/19/2017 at 8:23 PM, hatetane said:[Edited link out]
Look at what one of our very own forum users has been up too!
Great job - very much appreciated.
who is that?
On 4/19/2017 at 2:10 PM, QuietSoldier said:Has anyone just stopped taking supplements for an extended period of time?
Im starting to notice alot of different supplements having similar effects. From b12, to vitamin D, zinc and numerous others. there is not one supplement I still take to this day since day 1 many years ago since looking into supplements, and I think that says alot. I believe there is some type of chronic inflammation going on in and around the stomach, that depending on what you take might just worsen this. One thought is for some reason there is increased acid production being generated by alot of different supplements that might already be chronically increased due to accutane. This could trigger a immune type response that could effect the whole body in various ways. Still looking into this.
Finally had my blood work done yesterday (I know it's been a while). Will post my reports as soon as I get them
I know elohel talked about this once before but does anyone else not get sick anymore? Like not even catching little colds? I'd like to know because I read something somewhere that stated high levels of testosterone cause men to get sick more often and I was wondering if there was some sort of correlation. Thoughts?
42 minutes ago, Colinboko said:I know elohel talked about this once before but does anyone else not get sick anymore? Like not even catching little colds? I'd like to know because I read something somewhere that stated high levels of testosterone cause men to get sick more often and I was wondering if there was some sort of correlation. Thoughts?
Not really sick in the traditional sense no. But people have had ongoing issues with sinuses, ear fullness, breathing,headaches, so its hard to tell what qualifies as sick anymore. Not sure if this correlates with testosterone, i've hit fairly T high numbers in the past, not supplementing anything.
3 minutes ago, guitarman01 said:49 minutes ago, Colinboko said:I know elohel talked about this once before but does anyone else not get sick anymore? Like not even catching little colds? I'd like to know because I read something somewhere that stated high levels of testosterone cause men to get sick more often and I was wondering if there was some sort of correlation. Thoughts?
Not really sick in the traditional sense no. But people have had ongoing issues with sinuses, ear fullness, breathing,headaches, so its hard to tell what qualifies as sick anymore. Not sure if this correlates with testosterone, i've hit fairly T high numbers in the past, not supplementing anything.
I mean I just feel like I know when I have a cold and what it feels like. So aside from my side effects making me feel out of it and like shit, I find it interesting that we can't really catch the common flu, stomach bug etc.
over active immune system?
anyone even hitting over 600 pg/ml of vitamin b12 in their blood tests?
My highest is maybe around 550 pq/ml, my lowest was in the 400s
I just posted that study how accutane reduces b12 levels. maybe my normal pre-accutane used to be alot higher then 550. alot of these people are hitting in the 1000s for b12 serum levels.
http://freshbeetle.com/vitamin-b12-blood-test-levels/
epigenetic modification may regulate the expression of GNMT
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356423/
Nutritional and hormonal modulation of glycine N-methyltransferase: implications for aberrant methyl group metabolism
Bunch of information on Retinoids effects on here.
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1813&context=rtd
You guys got this gene mutation? I do.
affects b12 transport and serum levels
frequency is only 4.4 percent of being a carrier
more info
| 4.4% | Frequency |
5 hours ago, guitarman01 said:You guys got this gene mutation? I do.
affects b12 transport and serum levels
frequency is only 4.4 percent of being a carrierGenome-wide significant predictors of metabolites in the one-carbon metabolism pathway. G allele associated with lower serum Vitamin B12. 'The TCN1 rs526934 G variant may reduce transport of cobalamin, resulting in lower plasma vitamin B12 levels.' [PharmGKB:Non-Curated GWAS results: Genome-wide Association Study of Vitamin B6, Vitamin B12, Folate, and Homocysteine Blood Concentrations. (Initial Sample Size: 2,934 individuals; Replication Sample Size: 686 individuals); (Region: 11q12.1; Reported Gene(s): TCN1; Risk Allele: rs526934-A); (p-value= 0.000002).This variant is associated with Folate pathway vitamins.] [GWAS:Folate pathway vitamins]
more info
4.4% Frequency
Thx for sharing!!
Were you born with this mutation or is the mutation a result of being on Accutane??
Or is it exacerbated by Accutane?, this is what I'd like to know!
5 hours ago, TrueJustice said:Were you born with this mutation or is the mutation a result of being on Accutane??
Or is it exacerbated by Accutane?,
I would say this is could be insignificant unless a couple other people had this same mutation. Then it could be very significant. What was your b12 level btw? Did you ever have this tested?
18 hours ago, guitarman01 said:anyone even hitting over 600 pg/ml of vitamin b12 in their blood tests?
My highest is maybe around 550 pq/ml, my lowest was in the 400s
I just posted that study how accutane reduces b12 levels. maybe my normal pre-accutane used to be alot higher then 550. alot of these people are hitting in the 1000s for b12 serum levels.
http://freshbeetle.com/vitamin-b12-blood-test-levels/
520. Range 180 up to 1100 pg/ml Is that too low for a 20 year old. I guess it would be better to be nearer the top of the range.
57 minutes ago, hatetane said:19 hours ago, guitarman01 said:anyone even hitting over 600 pg/ml of vitamin b12 in their blood tests?
My highest is maybe around 550 pq/ml, my lowest was in the 400s
I just posted that study how accutane reduces b12 levels. maybe my normal pre-accutane used to be alot higher then 550. alot of these people are hitting in the 1000s for b12 serum levels.
http://freshbeetle.com/vitamin-b12-blood-test-levels/
520. Range 180 up to 1100 pg/ml Is that too low for a 20 year old. I guess it would be better to be nearer the top of the range.
One Japanese study states the normal limits as 5001,300 pg/mL.[42]
I would like to look at a lot more serum results in the normal population at different ages and male/female compared to post accutane users.
I would like to know how this blood level would and should normally respond to oral supplements.
36 minutes ago, guitarman01 said:One Japanese study states the normal limits as 5001,300 pg/mL.[42]
I would like to look at a lot more serum results in the normal population at different ages and male/female compared to post accutane users.
I would like to know how this blood level would and should normally respond to oral supplements.
I know metformin reduced a level of over 600 to 320 in two months of taking meds (a friend) and the doctors never once mentioned that bloods would be necessary on a regular basis. It was a pharmacist that advised a blood test. My friend was already experiencing daily debilitating migraines - charming!!
This is a must read guys.
http://ahrp.org/thalidomide-litigation-grunenthal-intimidation-tactics-landmark-settlement/
Seriously, what hope have we got.
Thousands of thalidomide victims every year still! 50 years of profit above the health of humans.
What a disgrace!
On 4/19/2017 at 0:16 PM, hatetane said:On 4/10/2017 at 2:06 AM, hatetane said:Time to be proactive!!
There is going to be a review of accutane in JUNE this year.
I want each and everyone of you from Europe to write the best email you can.
Send it to the representatives of your country and to as many independents as possible.
For those of you outside of the UK please send to all independents and to as many others as you can especially
to the MHRA in the UK.
This as a minimum, everyone send to as many representatives as you can. Once you have composed an email it is simply a matter of re-sending.
You must include your name ( data protection will protect you) age, dosage, duration and how long post A.
A complete and thorough list of all your side effects.
You must tell your story about how and when your sides evolved.
Write about your full experience but I especially ask that you concentrate on sexual dysfunction (brain - penis disconnect) and any sides that are not listed on the PIL.
For anyone who has or still is - talk about depression and try and describe it. I believe that many doctors think you are all depressed because of your acne and you really have to convey how this unique depression gets hold of you and how long it lasts.
For those of you who feel they have had there personalties change and or depersonalisation please describe it.
Most importantly talk about any suicidal ideation or attempts.
Please mention how accutane has effected and or interrupted your study/work.
Mention how you feel about accutane and any regrets you might have about taking it.
Write about any recovery and the time this has taken.
Add a brief detail about the response of you doctors and finish of with:
1 I have reported my sides to .......
2 I have never reported my side effects.This is your opportunity to protest and have your voice heard. It will probably take you an hour which is less time than it would take to visit you GP.
I want you all to post on here when you have done it so as to encourage others to participate.
This is not a lot to ask, no excuses are acceptable .
We are trying to get better 'Patient Information' for anyone being giving accutane in the future - if you feel stronger than this then say so.Please, Please participate.
http://www.ema.europa.eu/ema/index.jsp?curl=pages/contacts/PRAC/people_listing_000112.jsp#MNMSPlease copy and paste on any other forums you use and let us know that you have done this.
Many many thanks.
Who is participating - PM me please
i am going to keep bumping this guys in the hope that you will all act!! Just a email - can't be too difficult can it!!!