Five bioactives (Manuka tree oil, lavender essential oil, green tea extract, olive leavesextracts and propolis) were screened using a combination of methods. All the bioactives demonstrated antibacterial activity against the growth of P. acnes and only green tea extract and Manuka tree oil had bactericidal ability to kill the bacterium (Figure 4.47). The killing rate of GTE was greater than MTO. When GTE was combined with honey, and MTO was combined with honey, the killing effects against P. acnes were higher than the bioactives used singly. The antibacterial activity of formulated creams containing honey, MTO and GTE were studied separately and in combination. The creams containing 10 % honey demonstrated bacteriostatic ability. Creams containing MTO (0.125 - 0.5 %) and GTE (0.125 1 %) exhibited bactericidal ability against P. acnes and the cell counts decreased rapidly. Cream containing 10 % honey and 1 % GTE caused approximately 5 log reductions in cell numbers; cream containing 10 % honey and 0.125 % MTO caused about 2 log reductions in cell numbers. No bacterial cells were detected at 10-2 dilutionwere found in the cream containing honey (10 %), MTO (0.125 %) and GTE (1 %). It is therefore evident that the honey containing both MTO and GTE had the most bactericidal ability against P. acnes.