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I saw some initial improvements when I was using it once per day and then I increased to twice per day as the regiment suggests. There are occasional days where I will skip one of the two and i do find that I notice a difference in my skin. I would say go by trial and error. If you find an improvement on once per day, why bump up to twice? however, if you arent' getting the clear skin you want, then you won't really know whether it works until you try twice per day for a significant period of time. The only downfall to trying once per day is that it will probably take you longer to know whether the regimen is working.